Journal: Molecules

Article Title: Determination of Pharmaceuticals, Heavy Metals, and Oxysterols in Fish Muscle

doi: 10.3390/molecules26051229

Figure Lengend Snippet: Pharmaceuticals and selected metabolites which were not detected in fish muscle with their limit of detection (ng/g).

Article Snippet: Acetaminophen D4, caffeine D9, salbutamol D7, trimethoprim D9, fluoxetine D5, bisoprolol D5, ciprofloxacin D8, mycophenolic acid D3, clindamycin D3, valsartan D3, erythromycin C13D3, dulfamethoxazole D4 were used as internal standards and were purchased from Toronto Research Chemicals (Toronto, Ontario, ON, Canada).

Techniques:

Journal: ACS Central Science

Article Title: Immunomodulatory Nanoparticles Enable Combination Therapies To Enhance Disease Prevention and Flare Control in Rheumatoid Arthritis

doi: 10.1021/acscentsci.5c00723

Figure Lengend Snippet: Synthesis and characterization of Agg-CLNP formulation. (a) Experimental schematic and chemical structure of aggrecan peptide conjugated calcitriol loaded nanoparticle synthesis. (b) Dynamic light scattering by intensity graph of Agg-CLNP formulation. (c) Dynamic light scattering by intensity plots of Agg-CLNP 7, 14, 21, and 28 days post synthesis. (d) Calcitriol content in Agg-CLNP 0, 7, 14, 21, and 28 days post synthesis as assessed by HPLC. (e) Calcitriol concentration in plasma at indicated time points and in untreated mice. Data in (d) are means ± SD of three technical replicates and in (e) are means ± SD of three experimental replicates. Statistical analyses in (e) were performed using ANOVA with Tukey’s multiple comparison test. Schematic in (a) was composed using BioRender and ChemDraw.

Article Snippet: Calcitriol ELISA kit (NBP2-82432) was purchased from Novus Biologicals.

Techniques: Formulation, Concentration Assay, Clinical Proteomics, Comparison

Journal: ACS Central Science

Article Title: Immunomodulatory Nanoparticles Enable Combination Therapies To Enhance Disease Prevention and Flare Control in Rheumatoid Arthritis

doi: 10.1021/acscentsci.5c00723

Figure Lengend Snippet: Agg-CLNP imparts an immunomodulatory phenotype on healthy human MDC in vitro . (a) Experimental schematic of MDC culture. (b and c) Proinflammatory cytokine concentrations in cell culture as measured by (b) IL-6 and (c) TNF. (d–h) Costimulatory molecules (CD40, CD80, and CD86), HLA-2, and CTLA-4 positivity on CD11c + MDC after culture with LPS only, free calcitriol, free dexamethasone, Agg-CLNP and a combination of dexamethasone with Agg-CLNP as measured by (d) CD40, (e) CD80, (f) CD86, (g) HLA-2, and (h) CTLA-4. Data in (b–h) are means ± SD of six technical replicates from representative experiments. Statistical analyses in (b–h) were performed using ANOVA with Tukey’s multiple comparison test. Schematic in (a) was composed using BioRender.

Article Snippet: Calcitriol ELISA kit (NBP2-82432) was purchased from Novus Biologicals.

Techniques: In Vitro, Cell Culture, Comparison